Class I hydrophobins pretreatment stimulates PETase for monomers recycling of waste PETs

Puspitasari, Nathania, Tsai, Shen-Long and Lee, Cheng-Kang (2021) Class I hydrophobins pretreatment stimulates PETase for monomers recycling of waste PETs. Class I hydrophobins pretreatment stimulates PETase for monomers recycling of waste PETs, 176. pp. 157-164. ISSN pISSN: 0141-8130, eISSN:1879-0003,Jurnal Internasional Bereputasi SJR(2020): 0.93, Q1, H-Index: 144

	Text (Class I hydrophobins pretreatment stimulates PETase for monomers recycling of waste PETs) 2-Class_I_hydrophobins_pretreatment_(Nathania).pdf Download (1MB)
	Text (Class I hydrophobins pretreatment stimulates PETase for monomers recycling of waste PETs_peer_review_) 2-R1&2-Class_I_hydrophobins_pretreatment_.pdf Download (1MB)
	Text (Class I hydrophobins pretreatment stimulates PETase for monomers recycling of waste PETs) 2-Class_I_hydrophobins_pretreatment_hasil cek similarity.pdf Download (2MB)

Abstract

Poly(ethylene terephthalate) hydrolase (PETase) from Ideonella sakaiensis 201-F6 was expressed and purified from Escherichia coli to hydrolyze poly(ethylene terephthalate) (PET) fibers waste for its monomers recycling. Hydrolysis carried out at pH 8 and 30 °C was found to be the optimal condition based on measured monomer mono (2-hydroxyethyl) terephthalate (MHET) and terephthalic acid (TPA) concentrations after 24 h reaction. The intermediate product bis(2-hydroxyethyl) terephthalate (BHET) was a good substrate for PETase because BHET released from PET hydrolysis was efficiently converted into MHET. Only a trace amount of MHET could be further hydrolyzed to TPA. Class I hydrophobins RolA from Aspergillus oryzae and HGFI from Grifola frondosa were expressed and purified from E. coli to pretreat PET surface for accelerating PETase hydrolysis against PET. The weight loss of hydrolyzed PET increased from approximately 18% to 34% after hydrophobins pretreatment. The releases of TPA andMHET fromHGFI-pretreated PETwere enhanced 48% and 62%, respectively. The selectivity (TPA/MHET ratio) of the hydrolysis reaction was approximately 0.5. ©

Item Type:	Article
Additional Information:	Jurnal Internasional Bereputasi SJR(2020): 0.93, Q1, H-Index: 144
Uncontrolled Keywords:	Hydrophobin PETase PET recycling
Subjects:	Engineering > Chemical Engineering
Divisions:	Journal Publication
Depositing User:	F.X. Hadi
Date Deposited:	19 Aug 2022 05:31
Last Modified:	13 Sep 2022 04:20
URI:	http://repository.ukwms.ac.id/id/eprint/31911

Actions (login required)

View Item