Ethanolic extract Ocimum sanctum Linn. induces an apoptosis in human lung adenocarcinoma (A549) cells

Wihadmadyatamib, Hevi, Karnati, Srikanth, Hening, Puspa, Tjahjono, Yudy, Rizal, Rizal, Maharjanti, Fitriana, Kusindarta,, Dwi Liliek, Triyono, Teguh and Supriatno, Supriatno (2019) Ethanolic extract Ocimum sanctum Linn. induces an apoptosis in human lung adenocarcinoma (A549) cells. Ethanolic extract Ocimum sanctum Linn. induces an apoptosis in human lung adenocarcinoma (A549) cells, 5 (11). pp. 1-9. ISSN 2435-8440

	Text (Ethanolic extract Ocimum sanctum Linn. induces an apoptosis in human lung adenocarcinoma (A549) cells) 1-Ethanolic_extract_ocimum_.pdf Download (1MB)
	Text (Ethanolic extract Ocimum sanctum Linn. induces an apoptosis in human lung adenocarcinoma (A549) cells) 1-Ethanolic_extract_ocimum_peer_review_.pdf Download (1MB)
	Text (Ethanolic extract Ocimum sanctum Linn. induces an apoptosis in human lung adenocarcinoma (A549) cells) 1-Ethanolic_extract_ocimum_Hasil Cek Similarity.pdf Download (3MB)

Abstract

Ocimum sanctum (OS) is tropical herbal plant which is easy to find and widely used as a vegetable food in Indonesia. In last decade, lung adenocarcinoma was in top position as male cancer disease in Indonesia. Recently, emerging data showing the extracts of different species of Ocimum exhibiting the anti-tumor properties. Further studies on lung lewis carcinoma demonstrated pro-apoptosis effects after the treatment with Ocimum extracts. However, the effect of OS of Indonesian origin in human alveolar pulmonary adenocarcinoma A549 cells remain unclear. Therefore, we aimed to investigate effects of ethanolic extract OS (EEOS) in A549 cell culture systems. Cell adhesion and viability assays revealed that EEOS significantly decreased the attachment into extracellular matrix of A549 cells. Morphological examination AO/EB and DAPI staining indicated that EEOS induced the cells shrinkage, DNA fragmentation and condensation of A549 cells. Further, EEOS treatment induced the apoptosis rate followed by up-regulation of reactive oxygen species (ROS), caspase-3 expression and decreased antiapoptotic protein Bcl-2. This condition also suppressed the expression of SOD2 as well as the GPx. In conclusion, our findings indicate that EEOS suppressed the viability of A549 cells, which may result from the activation of ROS promoting the apoptosis signaling via mitochondrial intrinsic pathway. Taken together, EEOS might be a good therapeutic potential to further understand its properties in the treatment of lung carcinoma

Item Type:	Article
Uncontrolled Keywords:	Food science Cell biology A549 ethanolic extract Ocimum sanctum Detachment Apoptosis
Subjects:	Pharmacy
Divisions:	Journal Publication
Depositing User:	F.X. Hadi
Date Deposited:	09 Aug 2021 04:40
Last Modified:	09 Aug 2021 04:40
URI:	http://repository.ukwms.ac.id/id/eprint/25639

Actions (login required)

View Item