Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica

Do, Quy Diem, Angkawijaya, Artik Elisa, Tran-Nguyen, Phuong Lan, Huynh, Lien Huong, Soetaredjo, Felycia Edi, Ismadji, Suryadi and Ju, Yi-Hsu (2014) Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22. pp. 296-302. ISSN 1021–9498

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Abstract

Limnophila aromatic is commonly used as a spice and a medicinal herb in Southeast Asia. In this study, water and various concentrations (50%, 75%, and 100%) of methanol, ethanol, and acetone in water were used as solvent in the extraction of L. aromatica. The antioxidant activity, total phenolic content, and total flavonoid content of the freeze-dried L. aromatic extracts were investigated using various in vitro assays. The extract obtained by 100% ethanol showed the highest total antioxidant activity, reducing power and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity. The same extract also exhibited the highest phenolic content (40.5 mg gallic acid equivalent/g of defatted L. aromatic) and the highest flavonoid content (31.11 mg quercetin equivalent/g of defatted L. aromatic). The highest extraction yield was obtained by using 50% aqueous acetone. These results indicate that L. aromatica can be used in dietary applications with a potential to reduce oxidative stress.

Item Type: Article
Uncontrolled Keywords: Antioxidants activity Effect of solvent Food analysis Limnophila aromatic Total phenol content
Subjects: Engineering > Chemical Engineering
Divisions: Faculty of Engineering > Chemical Engineering Study Program
Depositing User: Felycia Edi Soetaredjo
Date Deposited: 17 Jul 2017 07:48
Last Modified: 22 May 2018 09:33
URI: http://repository.ukwms.ac.id/id/eprint/11256

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