Utilization of nanomaterial with cubic mesostructure to increase efficiency of enzymatic conversion of cellulose into glucose

Harmoko, Christian and Sucipto, Kevin Indrawan (2015) Utilization of nanomaterial with cubic mesostructure to increase efficiency of enzymatic conversion of cellulose into glucose. Working Paper (Laporan Penelitian Laboratorium (FT-Kimia)). Faculty of Engineering. (Unpublished)

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The use of conventional fuel becomes major part of life that can’t be separate anymore. However, conventional fuel can’t be renewable so over time the stocks are dwindling and cause fuels price has been increase. Recently, Indonesia has changed to become one of petroleum importing countries. It makes the country economic more vulnerable against rising fuel prices. Availability of alternative fuels such as bio-ethanol or bio-diesel becomes very important. To overcome this challenges, alternative fuel researches are encouraged by so many countries. One of organic component that can be used as a raw material to produce bio-ethanol is cellulose. Cellulose is the largest organic material in earth. Cellulose can be easily founded from agricultural residue such as corn head. Cellulose can be converted to glucose which is the base material to produce bio-ethanol. There are two processes to convert cellulose into glucose: through chemical process and enzymatic process. Cellulase enzymes convert cellulose materials into glucose. The enzymatic process have a benefit, the high cellulose conversion into glucose and zero waste process as compared to chemical process. Yet, the enzymatic process is hindered due to the high cost of enzyme production. For this reason, the increase in enzymatic process efficiency is unavoidable to cut production cost. On this study, we intend to improve efficiency from enzyme with nanoporous material for reusability from cellulose enzyme, while keeping activity and stability from enzyme. The purpose from this research is to study the use of nanomaterial to improve enzyme stability and the use of nanomaterial to enzyme reusability. There are many factors that influence activity, stability and reusability from cellulase enzyme such as chemical character of surface, particle size from nanoporous material (micron sized: 4-8 micrometer and submicron : 100-300 nm), property of nanoporous will be examined to reduce production cost so it can increase efficiency from enzymatic process. This research is divided into 4 stages, nanoporous silica synthesis, chemical character modification of surface, enzyme immobilization, and activity, stability and reusability of enzyme measurement. At first stage, cubic mesostucture nanoporous silica (FDU-12) will be synthesized in two different particle size: micron size and submicron size. Then, chemical character modification of surface with organosilane (VTMS: Vinyltrimetoxysilane) in different ratio. Next, enzyme immobilization and measure enzyme activity with: filter paper and CMC methods. Functionalized nanoporous silica product that filled by enzyme is used to convert cellulose into glucose. Finally the optimum condition that give best efficiency to improve activity, stability and reusability from cellulose enzyme will be determined.

Item Type: Monograph (Working Paper (Laporan Penelitian Laboratorium (FT-Kimia)))
Department: ["eprint_fieldopt_department_Faculty of Engineering" not defined]
Uncontrolled Keywords: enzyme immobilization, cellulase enzyme, mesoporous silica, nanoparticles, surface modification
Subjects: Engineering > Chemical Engineering
Divisions: Faculty of Engineering > Chemical Engineering Study Program
Depositing User: Users 2628 not found.
Date Deposited: 22 Jan 2016 07:54
Last Modified: 25 Jan 2016 04:35
URI: http://repository.ukwms.ac.id/id/eprint/4361

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