Detection of Chloramphenicol Residue in Shrimp (Penaeus Monodon) By High Performance Liquid Chromatography

Caroline, Caroline, Esar, . Senny Yesery and Foe, Kuncoro (2010) Detection of Chloramphenicol Residue in Shrimp (Penaeus Monodon) By High Performance Liquid Chromatography. In: Detection of Chloramphenicol Residue in Shrimp (Penaeus Monodon) By High Performance Liquid Chromatography., 21-22 July 2010, Surabaya.

Text (Detection of Chloramphenicol Residue in Shrimp (Penaeus Monodon) By High Performance Liquid Chromatography) 18pi-Study_of_clove_leaves_.pdf Download (5MB)

Abstract

In spite of its potential toxicity, chloramphenicol at its therapeutic dose is used for the treatment of various infections in human. Since chloramphenicol is used as a preservative in food producing animals and animal feed products, many countries have made a strict regulation to control the residual content of chloramphenicol in meat, including shrimp meat (Penaeus monodon). The aim of this study was to develop a method for the detection of chloramphenicol in shrimp meat at the part per billion (ppb) level. The shrimp meat was homogenized in saline buffer, extracted using chloroform, evaporated and then reconstituted in the mobile phase. The resulting solution was injected onto an HPLC-C18 column and detected at 265 nm. A mixture of methanol: water (30:70, v/v) was used as a mobile phase. A linear curve of the standard solution ranging between 0.10 and 1.0 μg/ml was constructed. The overall recovery of analyte was found to be 82.77 (± 3.17%). The limit of quantitation (LOQ) and the limit of detection (LOD) of chloramphenicol were 0.28 ng per g of shrimp (0.28 ppb) and 0.09 ppb, respectively.

Item Type:	Conference or Workshop Item (Poster)
Uncontrolled Keywords:	chloramphenicol, shrimp meat, HPLC
Subjects:	Pharmacy
Divisions:	Proceeding
Depositing User:	F.X. Hadi
Date Deposited:	04 Mar 2024 03:27
Last Modified:	04 Mar 2024 03:27
URI:	http://repository.ukwms.ac.id/id/eprint/39232

Actions (login required)

View Item