Encapsulation of β‑Glucosidase within PVA Fibers by CCD-RSMGuided Coelectrospinning: A Novel Approach for Specific Mogroside Sweetener Production

Virly, Virly, Chiu, Chun-Hui, Tsai, Tsan-Yu, Yeh, Yi-Cheun and Wang, Reuben (2020) Encapsulation of β‑Glucosidase within PVA Fibers by CCD-RSMGuided Coelectrospinning: A Novel Approach for Specific Mogroside Sweetener Production. Encapsulation of β‑Glucosidase within PVA Fibers by CCD-RSMGuided Coelectrospinning: A Novel Approach for Specific Mogroside Sweetener Production, 68 (42). pp. 11790-11801. ISSN pISSN: 1520-5118, eISSN: 0021-8561

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Abstract

Siamenoside I is a rare mogroside in Siraitia grosvenorii Swingle and has become one of the target ingredients in natural sweetener production. However, the complex structure of siamenoside I has hindered its production in various ways. Here, a yeast cell that produces a specific β-glucosidase for siamenoside I conversion from mogroside V was constructed, and the enzymes were coelectrospun with poly(vinyl alcohol) followed by phenylboronic acid cross-linking to provide potential usage in the batch production process of Siamenoside I. A central composite design (CCD)-response surface methodology (RSM) was used to find the optimum coelectrospinning parameters. The pH stability and sodium dodecyl sulfate tolerance increased for the entrapped enzymes, and positive correlations between the fiber diameter and enzymatic activity were confirmed. The batch process showed an average siamenoside I production rate of 118 ± 0.08 mg L−1 h−1 per gram of fiber. This is the first research article showing specific siamenoside I production on enzyme-loaded electrospun fibers.

Item Type:	Article
Uncontrolled Keywords:	siamenoside I, β-glucosidase, poly(vinyl alcohol), coelectrospinning, CCD-RSM
Subjects:	Agriculture and Food Technology
Divisions:	Journal Publication
Depositing User:	F.X. Hadi
Date Deposited:	20 Jun 2023 06:28
Last Modified:	18 Jul 2023 01:32
URI:	http://repository.ukwms.ac.id/id/eprint/35086

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