Perbandingan kadar total fenol dari ekstrak dan fraksi soxhletasi cinnamomi cortex

Seriyani, Ninitania (2022) Perbandingan kadar total fenol dari ekstrak dan fraksi soxhletasi cinnamomi cortex. Undergraduate thesis, Widya Mandala Surabaya Catholic University.

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Abstract

Kayu manis (Cinnamomum burmannii) adalah salah satu rempah paling terkenal dan tertua di dunia. Ini memiliki banyak kegunaan sebagai bahan tambahan makanan, serta dalam pengobatan tradisional dan modern. Senyawa identitas kulit kayu manis adalah cinnamaldehyde (80-95%) minyak atsiri. Dari penelitian sebelumnya disajikan bahwa senyawa fenolik kayu manis terkait dengan berbagai aktivitasnya, meskipun kandungan kumarinnya menyebabkan toksisitas pada beberapa organ. Dengan demikian, fraksinasi untuk meningkatkan fenoliknya diusulkan dalam penelitian ini. Selain itu, penelitian ini juga untuk mengetahui rendemen ekstrak dan fraksinya yang dihasilkan dengan soxhletasi etanol (SC). Ekstrak dilanjutkan dengan ekstraksi cair-cair dengan pelarut n-heksana (SCH), etil asetat (SCE), dan sisa fraksi air (SCA). Kandungan fenolat total dilakukan dengan menggunakan metode Folin-Ciocalteu dengan microplate reader 96 sumur. Skrining fitokimia dilakukan dengan kromatografi lapis tipis (KLT) dengan silika gel F254 dan toluena-p:etil asetat (97:3). KLT menghasilkan kandungan alkaloid, flavonoid, tanin, fenol, kuinon, dan steroid. Hasil ekstrak dan fraksi diperoleh 24,266 ± 0,107% untuk SC, 2,303 ± 0,464% untuk SCH, 9,398 ± 0,322% untuk SCE. Sedangkan hasil kandungan fenol total berturut-turut adalah 55,762 ± 0,652 mg GAE/g ekstrak etanol, 35,251 ± 0,624 mg GAE/g etil asetat, 52,499 ± 0,957 mg GAE/g air, dan rutin 57,549 ± 0,631 mg GAE/g. Hasil ini memberikan informasi ilmiah untuk digunakan lebih lanjut. Kata kunci: Cinnamomi burmannii, soxhletasi, total fenol, KLT Cinnamon (Cinnamomum burmannii) is one of the most famous, and oldest spices in the world. It has many uses as food additive, as well as in traditional and modern medicine. The identity compound of Cinnamon bark is cinnamaldehyde (80-95%) volatile oil. From the previous study presented that Cinnamon’s phenolic compounds related to its various activities, though its coumarin contents caused toxicities to some organs. Thus, the fractionation to increased its phenolics was proposed in this research. Moreover, the study was also to determine their extract and fractions yield, which were produced by ethanolic soxhletation (SC). The extract was proceeded further with liquid-liquid extractions with n- hexane (SCH), ethyl acetate (SCE) solvents, and remain of water fractions (SCA). The total phenolics content was carried out by using Folin-Ciocalteu method with 96 well microplate reader. The phytochemical screening was done by thin layer chromatography (TLC) with silica gel F254 and toluene- p: ethyl acetate (97:3). The TLC resulted its contained alkaloids, flavonoids, tannins, phenols, quinones, and steroids. The extract and fractions yields were obtained 24.266 ± 0.107% for SC, 2.303 ± 0.464% for SCH, 9.398 ± 0.322% for SCE. Meanwhile the results of the total phenolic content were 55.762 ± 0.652 mg GAE/g ethanol extract, 35.251 ± 0.624 mg GAE/g ethyl acetate, 52.499 ± 0.957 mg GAE/g water, and rutin 57.549 ± 0.631 mg GAE/g, respectively. These results gave scientific information for its further used.

Item Type: Thesis (Undergraduate)
Department: S1 - Farmasi
Contributors:
Contribution
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Email
Thesis advisor
Ervina, Martha
NIDN0717027401
martha.pharm@ukwms.ac.id
Uncontrolled Keywords: Cinnamomi burmannii, soxhletasi, total fenol, KLT
Subjects: Pharmacy
Divisions: Faculty of Pharmacy > Pharmacy Study Program
Depositing User: Users 12381 not found.
Date Deposited: 03 Feb 2023 06:50
Last Modified: 03 Feb 2023 06:50
URI: http://repository.ukwms.ac.id/id/eprint/34117

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