Effect of Pholiota nameko Polysaccharides Inhibiting Methylglyoxal-Induced Glycation Damage In Vitro

Lin, His, Lin, Ting-Yun, Lin, Jer-An, Cheng, Kuan-Chen, Santoso, Shella Permatasari, Chou, Chun-Hsu and Hsieh, Chang-Wei (2021) Effect of Pholiota nameko Polysaccharides Inhibiting Methylglyoxal-Induced Glycation Damage In Vitro. Effect of Pholiota nameko Polysaccharides Inhibiting Methylglyoxal-Induced Glycation Damage In Vitro, 10 (10). p. 1589. ISSN 20763921, Jurnal Internasional Bereputasi SJR(2020): 1.07, Q2, H-Index: 46

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Abstract

Advanced glycation end products (AGEs) can induce oxidative stress and inflammation. AGEs are major risk factors for the development of many aging-related diseases, such as cancer and diabetes. In this study, Pholiota nameko polysaccharides (PNPs) were prepared from water extract of P. nameko via graded alcohol precipitation (40%, 60%, and 80% v/v). We explored the in vitro antiglycation ability of the PNPs and inhibition of methylglyoxal (MG)-induced Hs68 cell damage. In a bovine serum albumin (BSA) glycation system, PNPs significantly inhibited the formation of Amadori products. Fluorescence spectrophotometry revealed that the PNPs trapped MG and reduced MG-induced changes in functional groups (carbonyl and ε-NH2) in the BSA. Pretreating Hs68 cells with PNPs enhanced the cell survival rate and protected against MG-induced cell damage. This was due to decreased intracellular ROS content. PNPs thus mitigate skin cell damage and oxidative stress resulting from glycation stress, making them a potential raw material for antiagingrelated skincare products.

Item Type:	Article
Additional Information:	Jurnal Internasional Bereputasi SJR(2020): 1.07, Q2, H-Index: 46
Uncontrolled Keywords:	antiglycation; advanced glycation end products; glycation stress; Pholiota nameko; polysaccharide; human dermal fibroblasts; cell aging
Subjects:	Engineering > Chemical Engineering
Divisions:	Journal Publication
Depositing User:	F.X. Hadi
Date Deposited:	13 Jul 2022 08:31
Last Modified:	03 Aug 2022 01:29
URI:	http://repository.ukwms.ac.id/id/eprint/31471

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